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. Author manuscript; available in PMC: 2019 Sep 4.
Published in final edited form as: Anesth Analg. 2019 May;128(5):1013–1021. doi: 10.1213/ANE.0000000000004058

Figure 2.

Figure 2.

Expression of opioid receptors and toll-like receptor 4 (TLR4) on natural killer (NK) and K562 cells. Expression of the mRNA for each gene was normalized to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) using the 2−ΔΔCt method. Relative expression of each opioid receptor is shown as a percentage of expression of TLR4 on K562 cells. All genes were expressed on both NK cells and K562 cells, with the exception of the κ opioid receptor on K562 cells. n = 4 separate donors; mean ± SD. OPRD indicates delta opioid receptor; OPRK, kappa opioid receptor; OPRM, mu opioid receptor; ORL, nociceptin receptor.