KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-ADAM10 | Millipore | Cat# 422751 |
| anti-ADAM10 (clone EPR5622) | Abcam | Cat# ab124695 |
| anti-APP (clone G12A) | Pierce Custom Antibodies | this paper |
| anti-mouse sAPPα | Biolegend | Cat# 826801 |
| anti-sAPPβ | IBL | Cat# 18957 |
| anti-sAPPβ-swe | IBL | Cat# 10321 |
| anti-human sAPPα (clone 6E10) | Biolegend | Cat# 803015 |
| anti-total sAPP (clone 22C11) | Millipore | Cat# MAB348 |
| anti-Aβ (3D6) | Eli Lilly | N/A |
| anti-Aβ (6E10) | Biolegend | Cat# 803015 |
| anti-pan-actin (clone ACTN05) | Fisher Scientific | Cat# MS-1295-P0 |
| anti-Ataxin-1 (clone N76/8) | NeuroMab | Cat# 75-117 |
| anti-BACE1 (clone D10E5) | Cell Signaling Technology | Cat# 5606 |
| anti-BACE1 | Abcam | Cat# ab2077 |
| anti-CIC | Bethyl Laboratories | Cat# A301-204A |
| anti-doublecortin (DCX) | Santa Cruz Biotechnology | Cat# sc-8066 |
| anti-ETV4 | ProteinTech | Cat# 10684-1-AP |
| anti-ETV5 | ProteinTech | Cat# 13011-1-AP |
| anti-GAPDH (clone 6C5) | EMD Millipore | Cat# MAB374 |
| anti-GFAP | Dako | Cat# Z0334 |
| anti-Iba1 | Wako | Cat# 019-19741 |
| anti-Ki-67 | Acris Antibodies | Cat# DRM004 |
| anti-NeuN | EnCor Biotechnology | Cat# RPCA-FOX3 |
| anti-PCP4 | Atlas Antibodies | Cat# HPA005792 |
| anti-PS1 (clone D39D1) | Cell Signaling Technology | Cat# 5643 |
| anti-RGS14 | Neuromab | Cat# 75-170 |
| anti-STEP | Cell Signaling Technology | Cat# 4396 |
| anti-synaptoporin | Synaptic Systems | Cat# 102 002 |
| Biological Samples | ||
| Human DNA samples for Alzheimer's disease families | National Institute of Mental Health (NIMH) AD Genetics Initiative Study | Blacker et al. 2003 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Actinomycin D | Sigma | Cat# A9415 |
| 3,3’-diaminobenzidine (DAB) | Vector Laboratories | Cat# SK-4100 |
| Thioflavine S | Sigma-Aldrich | Cat# T1892 |
| Congo red | Sigma-Aldrich | Cat# C6277 |
| FD Cresyl Violet Solution | FD NeuroTechnologies | Cat# PS102-01 |
| Critical Commercial Assays | ||
| FD Rapid GolgiStain Kit, | FD NeuroTechnologies | Cat# PK401 |
| Human/Rat Abeta40 ELISA Kit | Wako | Cat# 294-62501 |
| Human/Rat Abeta42 ELISA Kit | Wako | Cat# 290-62601 |
| Click-iT Nascent RNA Capture Kit | ThermoFisher Scientific | Cat# C10365 |
| Dual Luciferase Reporter Assay System | Promega | Cat# E1910 |
| Congo Red Staining Kit | Sigma-Aldrich | Cat# HT60-1KT |
| qPCR assay for Bace1 mRNA expression | Applied Biosystems | Mm00478664_m1 |
| qPCR assay for Atxn1 mRNA expression | Applied Biosystems | Mm00485928_m1 |
| qPCR assay for Etv1 mRNA expression | Applied Biosystems | Mm00514804_m1 |
| qPCR assay for Etv4 mRNA expression | Applied Biosystems | Mm00476696_m1 |
| qPCR assay for Etv5 mRNA expression | Applied Biosystems | Mm00465816_m1 |
| qPCR assay for Rora mRNA expression | Applied Biosystems | Mm01173766_m1 |
| qPCR assay for Gfi1 mRNA expression | Applied Biosystems | Mm00515853_m1 |
| qPCR assay for Gapdh mRNA expression | Applied Biosystems | Mm99999915_g1 |
| Experimental Models: Cell Lines | ||
| Ataxin-1 WT and KO mouse embryonic fibroblasts | This paper | N/A |
| Ataxin-1 WT and KO mouse primary cortical neurons | This paper | N/A |
| HEK293T cells | ATCC | CRL-3216 |
| Experimental Models: Organisms/Strains | ||
| Ataxin-1 KO mice | Matilla et al., 1998 | N/A |
| Ataxin-1 154Q knock-in mice | Watase et al., 2002 | N/A |
| APPswe/PSEN1ΔE9 (APP-PS1) mice | The Jackson Laboratory | Stock # 34832-JAX |
| BACE1 KO mice | The Jackson Laboratory | Stock # 004714 |
| Oligonucleotides | ||
| Primers for Atxn1−/− mouse genotyping | primer1:CCCCTACCCACTCCTCATAT primer2:GGCCGTGATACAGTTTGCTG primer3:CCGGTGGATGTGGAATGTGT | |
| Primers for Bace1−/− mouse genotyping | WT_pr:AGGCAGCTTTGTGGAGATGGTG MT_pr:TGGATGTGGAATGTGTGCGAG Common_pr:CGGGAAATGGAAAGGCTACTCC | |
| Primers for APPswe-PSEN1ΔE9 mouse genotyping | Tg_fp:AATAGAGAACGGCAGGAGCA Tg_rp:GCCATGAGGGCACTAATCAT In_fp:CTAGGCCACAGAATTGAAAGATCT In_rp:GTAGGTGGAAATTCTAGCATCATCC | |
| Primers for Atxn1154Q/+ knockin mouse genotyping | KI_fp: GTGAGTTTGGGTCTGGCATC KI_rp: CCAAAAGTTAGGATCACAGCCC | |
| Primers for Bace1 qPCR with SYBR Green | fp:TCTTTTCCCTGCAGCTCTGT rp:ACTGCCCGTGTATAGCGAGT | |
| Primers for Gapdh qPCR with SYBR Green | fp:AGGTCGGTGTGAACGGATTTG rp:TGTAGACCATGTAGTTGAGGTCA | |
| Primers for BACE1 promoter cloning | fp:TAGCCCGGGCTCGAGAACCATACCGG CTTTTCTCCT rp:CGGAATGCCAAGCTTCCACCATAATCCAGCTCG | |
| Primers for ETV site mutagenesis | Mutation:TGGCAGGCAATCCGGCTCATAGGGCTGGAGAGGGGTCT Deletion:CCTCTCCAGCCCAGCCGGATTGCC | |
| Recombinant DNA | ||
| pGL3-Basic Vector | Promega | Cat# E1751 |
| pRL-TK | Promega | Cat# E2231 |
| pLX_TRC311 | Addgene | Cat# 113668 |
| pLX_TRC311 ETV4-S | Addgene | Cat# 74983 |
| pLX_TRC311 ETV4-L | Addgene | Cat# 74982 |
| pLX_TRC311 ETV5 | Addgene | Cat# 74984 |
| Software and Algorithms | ||
| ImageJ | https://imagej.nih.gov/ij/ | |
| Axoscope | Axon Instruments | N/A |
| Mini Analysis Program | Synaptosoft | N/A |
| Origin | Microcal Software | N/A |
| TRANSFAC | http://genexplain.com/transfac/ | |
| PROMO | http://alggen.lsi.upc.es/cgi-bin/promo_v3/promo/promoinit.cgi?dirDB=TF_8.3 | |
| Other | ||
| Nikon C2 confocal microscope | Micro Video Instruments, Inc. | N/A |
| Olympus IX70 confocal microscope | Olympus | 1X70 |
| Nikon Ci Upright microscope | Nikon | Ci-L |
| Touch Real-Time PCR Detection System | Bio-Rad | CFX96 |
| In-Fusion HD EcoDry Cloning Plus system | Clontech | Cat# 638915 |
| jetPrime transfection reagent | Polyplus transfection | Cat# 114-15 |
| QuikChange Lightning Multi Site-Directed Mutagenesis Kit | Agilent | Cat# 210513 |