Table 2. Primers Used for RT-qPCR Expression Analysis.
target | forward primer | reverse primer |
---|---|---|
Human ACTA1 set 1 | 5′- GAGGCTCAGAGCAAGAGAG | 5′- TCGTTGTAGAAGGTGTGGTG |
Human ACTA1 set 2 | 5′- GGAGCGCAAATACTCGGTG | 5′- CATTTGCGGTGGACGATGG |
Mouse Gtf2b | 5′- CTTCATGTCCAGGTTCTGCTCC | 5′- GGAACCAAGTCCAGCTCCAC |
Human DMPK | 5′- CACGTTTTGGATGCACTGAGAC | 5′- GATGGAGGGCCTTTTATTCGCG |
Human MBNL1 | 5′- CGCAGTTGGAGATAAATGGACG | 5′- CACCAGGCATCATGGCATTG |
Human MBNL2 | 5′- CCTGGTGCTCTTCATCCTTTAC | 5′- GTGAGAGCCTGCTGGTAGTG |
Human GAPDH | 5′- AATCCCATCACCATCTTCCA | 5′- TGGACTCCACGACGTACTCA |