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. 2019 Sep 4;14(9):e0221394. doi: 10.1371/journal.pone.0221394

Fig 2. Western blot analysis of different Pfs25-dS constructs in crude cell lysates before and after treatment with EndoH.

Fig 2

Crude cell lysates of three different recombinant H. polymorpha strains were analyzed by anti-dS (mAb 7C12) and anti-Pfs25 (mAb 32F81) Western blots. The strains co-expressed the wildtype dS and one of the three Pfs25-dS fusion protein constructs: “M” (N-terminal artificial start-methionine), “CL” (N-terminal chicken lysozyme signal sequence) or “QQ” (N-terminal chicken lysozyme signal sequence and N112Q, N187Q aa exchanges). Samples were treated with EndoH where indicated. M: molecular weight marker.