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. 2019 Sep 4;14(9):e0221394. doi: 10.1371/journal.pone.0221394

Fig 3. Analyses of purified Pfs230c-dS/dS VLP derived from strain RK#114.

Fig 3

(A): Western blot probed with anti-dS 7C12 mAb (lane 1) or probed with polyclonal anti-Pfs230 antibody (lane 2) and Coomassie stained PAA gel (lanes 3–10). Samples loaded in lanes 5–8 were treated with an EndoH. Lane 9: purified plain dS VLP. Lane 10: EndoH used. M: molecular weight marker. (B): TEM imaging at 100,000-fold magnification. (C): N-SIM analysis of purified VLP containing Pfs230c antigen showing immunolabeling of dS (green) or Pfs230c (red). Three nano-scaled spots that showed co-localization of the fluorescence markers (yellow) were representatively circled. (D): Size distribution determined by DLS.