Table 1. List of immunoreagents used for specific detection of the target proteins.

Antigen	Primary antibody	Source	Reference
dS	7C12(a)	BioGenes GmbH (Berlin, Germany)	[56,57]
Pfs25	32F81(b)	National Institutes of Health (NIH, Bethesda, Maryland, USA)	[9,58]
	4B7(b)	National Institutes of Health (NIH, Bethesda, Maryland, USA)	[23,59]
Pfs230	mouse polyclonal(c)	National Institutes of Health (NIH, Bethesda, Maryland, USA)	[55]
	1B3	National Institutes of Health (NIH, Bethesda, Maryland, USA)	[10]

^(a) detects the wild-type dS and the dS domain of each fusion protein

^(b) Monoclonal antibodies 32F81 and 4B7 were kindly provided by PATH Malaria Vaccine Initiative and BEI Resources NIAID, NIH.

^(c) Polyclonal mouse antibody was kindly provided by Carole Long and Kazutoyo Miura, NIH.