Table 2. Summary of fusion proteins constructed and recombinantly produced in H. polymorpha.
| Fusion protein | Number of aa(a) |
MW [kDa] |
Genbank reference of CDS | P. falciparum donor protein (Genbank reference) | Donor protein domain fused to dS | aa linker between P. falciparum antigen and dS |
|---|---|---|---|---|---|---|
| Pfs25-dS | 341 | 37.0 | MH142260 | zygote/ookinete surface protein Pfs25 (XP_001347587.1) | aa 23–193 [25] | GAGA |
| Pfs230c-dS | 861 | 98.0 | MH142261 | gametocyte surface protein Pfs230 (XP_001349600.1) | aa 443–1132 [22] | GAGA |
| Pfs230D1M-dS | 366 | 40.3 | MH142262 | gametocyte surface protein Pfs230 (XP_001349600.1) | aa 542-736(b) [38] | GAGA |
(a) Including an artificial start-methionine and aa 2–167 of the dS at the C-terminus
(b) Including N585Q aa exchange, elimination of a potential N-glycosylation site