Figure 1. Representative scheme of the minimal binary vector for fungi transformation through ATMT. (A) Design of the plasmid showing all the minimal modules that compose it, including the four versions of the expression cassette that were constructed. pLUO-Green1 (with eGFP) and pLUO-Red1 (with mCherry) have a Pura3 promoter for hph expression, while pLUO-Green2 and pLUO-Red2 uses Prp2 from T. reesei. (B) Representation of the restriction enzymes available in the MCS for several cloning options.