Table 1. Primers used in this study.
| Name | Sequence (5’- 3’) | Target DNA |
|---|---|---|
| 5’_GFP_HindIII | GCGCAAGCTTCGGTATCGATCATGAGTAAAG | pMCB17-apx |
| 3’_GFP | TTAAGCCGGCGCGCC | pMCB17-apx |
| 5’_Tcyc_GFP | GGCGCGCCGGCTTAACTCCTCCCACATCCGC | S. cerevisae |
| 3’_Tcyc_SpeI_BamHI | GCGCGGATCCACTAGTAAGCCTTCGAGCGTCCC | S. cerevisae |
| 5’_hph | CTGACACTAGCGCCACC | pGL4.14 |
| 3’_hph | GTTTAAACTCGACCTACCTCC | pGL4.14 |
| 5’_Pura3_XbaI | GCGCTCTAGAGTGCACCATACCACAGC | pRS42614 |
| 3’_Pura3_hph | TGGCGCTAGTGTCAGTGAGATTTATCTTCGTTTCCTGC | pRS42614 |
| 5’_Tadh1_hph | AGGTCGAGTTTAAACGGTAGATACGTTGTTGACAC | S. cerevisae |
| 3’_Tadh1_SpeI_EcoRI | GCGCGAATTCACTAGTGTGGTCAATAAGAGCGACC | S. cerevisae |
| 5’_LB_GFP_PacI | GCGCTTAATTAATGGCAGGATATATTGTGGTGTAAACATAACAATTTCACACAGGACCTAGG | pLUO |
| 3’_RB_Tadh1_SpeI | GCGCACTAGTGTTTACCCGCCAATATATCCTGTCAGTGGTCAATAAGAGCGACC | pLUO |
| 5’_Tcyc | CTCCTCCCACATCCGC | pLUO |
| 3’_Tcyc | AAGCCTTCGAGCGTCC | pLUO |
| 5’_HindIII_mCherry | GCGCAAGCTTGGTATGGTGAGCAAGGGC | pMR117 |
| 3’_mCherry_Tcyc | GGTTAGAGCGGATGTGGGAGGAGTTACTTGTACAGCTCGTCC | pMR117 |
| 5’_Tcyc_Prp2 | GGTTTTGGGACGCTCGAAGGCTTCGGCTGCGTGAACAGACG | T. reesei |
| 3’_Prp2_hph | GGTGGCGCTAGTGTCAGGTGGTTTGAGTTGGGTTGAGATAGG | T. reesei |
Sites for restriction enzymes are underlined.