Fig 2. Lactate production by self-renewing and differentiating cells.

Extra-cellular lactate concentration in self-renewing cells and cells induced to differentiate for 24h, 48h and 72h was assessed following incubation during 2h in fresh media. Represented is the ratio of extra-cellular lactate concentration of each condition divided by the self-renewal condition. Lactate concentration of self-renewing cells is equal to 1, as divided by itself, and indicated by the dotted line. Bars represent mean +/- S.D. calculated from four independent experiments for 24h and 48h differentiation time-points and five independent experiments for 72h and self-renewal. A t-test was applied to assess whether means were significantly different (*: p-value < 0.05; n.s.: non significant).