Fig 5. Dissection of cellular respiration during the differentiation process.

Different respiration parameters were assessed in self-renewing T2EC and T2EC induced to differentiate for 24, 48 and 72h. A: Routine respiration corresponds to oxygen consumption without any addition. B: Respiration dedicated to ATP production was evaluated by subtracting respiration after oligomycin treatment from routine respiration. C: Subtraction of routine respiration from maximal uncoupled respiration by FCCP reflected mitochondrial respiratory reserve. D: Cytochrome C oxydase activity was assessed by subtracting respiration following ascorbate addition from maximal TMPD-related respiration. Bars represent means +/- S.E.M. from five independent experiments performed in duplicates. A paired t-test was applied to assess whether means were significantly different (*: p-value < 0.05; n.s.: non significant).