Fig 8. Consequences of OXPHOS impairment on T2EC differentiation.

A: T2EC were incubated in differentiation media with antimycin A (10nM) or DMSO, during 48h. The differentiation rate was assessed using benzidine. The percentage of differentiating cells was calculated as the benzidine positive cells divided by the total cells. Results are the mean +/- S.D. from four independent experiments. The significance of the difference (p-value inf. to 0.05) between control and antimycin A-treated cells was calculated using a t-test. B: LDHA and betaglobin relative mRNA levels were quantified in T2EC treated with either antimycin A (10nM) or DMSO, during 48h in differentiation media. Data were normalized according to hnRNP and eif5 standard genes. The data shown are the mean fold changes +/- the standard deviation of gene expression under Antimycin A treatment with respect to the control (DMSO) condition, from three independent experiments. The red line represents fold change value y = 1. C: Mortality rate was compared between T2EC treated with antimycin A (10nM) in self-renewal (normal culture conditions) and differentiation media, for 48h. Mortality ratios were calculated as the trypan positive cell number over total cell number. The data shown are the mean +/- S.D. calculated from four independent experiments. An unilateral t-test was applied to assess whether means were significantly different (*: p-value < 0.05).