Fig. 4.

Inhibition of LPS-induced activation of Akt and STAT3 phosphorylation by LA-Dec. (A) BV2 cells were pre-treated with 30 μM of LA-Dec, LA, or Dec for 2 hours and stimulated with 0.1 μg/ml LPS. Total cell lysates were prepared at 30 minutes, 2 hours, or 6 hours, and the levels of unphosphorylated- and phosphorylated-ERK1/2, -p38, -JNK, -Akt, and -STAT3 were measured by Western blotting. (B) BV2 (upper panels) or RAW264.7 cells (lower panels) were pre-treated with 30 μM of LA-Dec, LA, or Dec for 2 hours and stimulated with 0.1 μg/ml LPS. Total cell lysates were prepared at 30 minutes, 2 hours or 6 hours. The levels of unphosphorylated- and phosphorylated-IκBα, Akt and STAT3 were examined by Western blotting by using specific antibodies. The blots are representative of three independent experiments.