Fig 1. Heatstroke model mice show severe BBB leakage.

(A) Vascular permeability, as measured by the leakage of Texas Red-dextran into the brain parenchyma (white arrows), was analyzed in brain tissue sections from normal mice (left) and heatstroke model mice (right). One representative image from five independent experiments is shown. Scale bar = 100 μm. (B) Expression of claudin-5 (Green) in brain microvascular endothelial cells (PECAM-1, Red) from normal mice (left) and heatstroke model mice (right) was evaluated by immunocytochemistry. One representative image from five independent experiments is shown. Scale bar = 100 μm. (C) Expression levels of claudin-5 and PECMA1 in brain microvessels from normal mice (n = 5) and heatstroke model mice (n = 5) were evaluated by western blotting. One representative image from two independent experiments is shown.