Fig 3. Expression of P-gp in iPS cell-derived brain microvascular endothelial cells is regulated by heat stress.

(A) iPS cell-derived microvascular endothelial cells on Transwell membranes were incubated at 37°C or 42°C for 12 h, and then the gene expression levels of MRP-1, BCRP, and P-gp were measured by quantitative RT-PCR. Gene expression levels at 37°C were set at 1.0. Data are the mean +/- S.D. from three independent experiments (n = 3). *P<0.05 (two-tailed Student’s t-test; compared with 37°C). (B) Expression levels of P-gp were evaluated by western blotting. One representative image from three independent experiments is shown.