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Wild-type and mutant constructs of tRNAphe. Tertiary interactions are depicted on the WT construct with yellow lines, based on PDB 1ehz. Mutant constructs (M1–M6) vary sequence and base pairing strength in the tunable region of the acceptor stem (shaded blue) and have ‘ACCA’ at the 3’ end. Base-pairing regions that are different between mutants and WT are in black boxes. Colors of WT and mutant are maintained in all display items.
