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. Author manuscript; available in PMC: 2019 Sep 4.
Published in final edited form as: Biochemistry. 2018 May 7;57(20):2994–3002. doi: 10.1021/acs.biochem.8b00345

Figure 2.

Figure 2.

WT and mutant thermal denaturation under in vivo-like solutions in the background of 2.0 mM free Mg2+. Each construct was globally fit every 2 nm between 250 and 290 nm. Thermal denaturation scans at 260 nm normalized using global fitting parameters in (A) buffer, (B) 20% PEG8000, (C) additional 14.0 mM Mg2+ weakly chelated to amino acids, and (D) 20% PEG8000 and additional 14.0 mM Mg2+ weakly chelated to amino acids. All four panels are in the background of 2.0 mM Mg2+ and 140 mM K+. Low temperature data was truncated in the fitting to avoid excess baselines, as is plotted above.