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Melting temperature and enthalpy of folding of WT and mutant tRNAs in 2.0 mM free Mg2+ with 20% PEG8000 with Mg2+-chelated amino acids. (A) Melting temperature and (B) ΔHfolding of tRNA and mutants in 20% PEG8000 and additional Mg2+-chelated amino acids. See Materials and Methods for the calculation of (pink) non-cooperative ΔH and (purple) fully cooperative ΔH limits.
