Information content of each position in tRNAphe. (A) The information bits for each position of tRNAphe. Positions are color coded as follows: tertiary contacts (green), secondary structure without tertiary contacts (pink), loops without tertiary contacts (blue), and the anticodon stem (purple). (B) Information content mapped onto the secondary structure of tRNA. Regions of high information content (1.76–2.00 bits), moderate information content (1.26–1.75 bits) and low information content (0.00–1.25 bits) are colored in red, orange, and black respectively. Joining lines between the D and TYC loops are passing behind the AC stem.