Figure 7.

Conceptual free energy diagram of WT and stabilizing mutants under dilute and in vivo-like conditions. (A) In dilute solution conditions folding is non-cooperative, and stabilization of secondary structure does not increase cooperativity because the penultimate state is also stabilized. (B) In more in vivo-like conditions folding is cooperative, and stabilization of secondary structure leads to an increase in cooperativity because the penultimate state is primary structure, which does not change in stability.