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. 2019 Aug 21;8:e44667. doi: 10.7554/eLife.44667

Figure 2. Novel serum-free primary astrocyte culture yields morphologically complex astrocytes.

Figure 2.

(A) Cartoon representation of magnetic separation of astrocytes for culture in serum-free, defined media. (B) Quantitative PCR data from cultured astrocytes at 7 and 14 DIV demonstrates purity of cultured cells. (C) mRNA expression of Ntrk2 isoforms in cultured astrocytes compared to age-matched acutely isolated astrocytes demonstrates a developmental upregulation of TrkB.T1 expression. (D) Representative image of GFAP and TrkB immunofluorescence shows localization of TrkB to astrocytic membrane. (E) Shape index (SI) and cumulative frequency analysis of astrocytes cultured in serum-containing or serum-free media and membranous immunolabeling demonstrates increased cellular complexity in serum-free conditions. (F) Representative images of GFAP+ astrocytes cultured for 14DIV in the presence or absence of serum, and representatives images of membrane (Glast)/GFAP staining. Data represented as mean + /- SEM, n = 3–6 cultures, with two wells collected per culture. Each data point in E represents an individual cell.