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. 2019 Aug 20;8:e47326. doi: 10.7554/eLife.47326

Figure 8. Mfsd2aa mutants exhibit increased transcytosis.

(A and B) TEM images of individual blood vessel cross-sections of adult wild-type (A) and mfsd2aa mutant fish (B). Endothelial cells (EC) are pseudo-colored green, pericytes (P) are pseudo-colored purple and red blood cells (RBC) are pseudo-colored red. (C and D) High-magnification images of endothelial cells outlined with white dashed lines of wild-type (C) and mfsd2aa mutants (D). The images are oriented with the lumen (L) on top and the ablumen (A) on the bottom. Mfsd2aa mutants appear to have normal tight junctions (green arrowhead) but elevated levels of luminal and abluminal vesicles (magenta arrows). The scale bars represent 200 nm. (E and F) Quantification of the vesicular densities both on the luminal (E) and abluminal (F) side of the endothelial cells reveals that mfsd2aa mutants have increased vesicular densities. N = 4 fish, each marked with a different color, with 15 blood vessels quantified for each fish and displayed as single points. ****p<0.0001, **p<0.01 by nested t test.

Figure 8—source data 1. Quantification of adult vesicular densities for mfsd2aa and mfsd2ab mutants.
elife-47326-fig8-data1.xlsx (14.1KB, xlsx)
DOI: 10.7554/eLife.47326.032

Figure 8.

Figure 8—figure supplement 1. Mfsd2ab mutants exhibit normal vascular maturation.

Figure 8—figure supplement 1.

(A and B) TEM images of individual blood vessel cross-sections of adult wild-type (A) and mfsd2ab mutants (B). Endothelial cells (EC) are pseudo-colored green, pericytes (P) are pseudo-colored purple and red blood cells (RBC) are pseudo-colored red when present in the lumen (L). (C and D) High-magnification images of endothelial cells outlined with white dashed lines of wild-type (C) and mfsd2ab mutants (D). The images are oriented with the lumen (L) on top and the ablumen (A) on the bottom. Mfsd2ab mutants appear to have normal tight junctions (green arrowhead) and levels of luminal and abluminal vesicles. The scale bars represent 200 nm. (E and F) Quantification of the vesicular densities both on the luminal (E) and abluminal (F) side of the endothelial cells reveals that mfsd2ab mutants have similar vesicular densities to wild-type siblings. N = 4 fish, each marked with a different color, with 15 blood vessels quantified for each fish and displayed as single points.