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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Acta Biomater. 2019 Jul 3;96:547–556. doi: 10.1016/j.actbio.2019.07.001

Fig. 6.

Fig. 6

Confocal microscopy study of the internalization of red-labeled pristine MSN and MSNConA in preformed E. coli biofilms after 90 min of incubation with 50 μg/mL of nanoparticles. 3D confocal reconstruction shows that bare MSNs are localized onto the biofilm surface, whereas MSNConA penetrate the biofilm and are placed at different depth levels. Live bacteria are stained in green (SYTO), nanoparticles in red (RhB), and the extracellular polysaccharide biofilm matrix in blue (Calcofluor). Scale bars, 20 µm.