Figure 6. Binding of zfBG_O-D1 and zfBG_O-D2 to TGF-β2.

A, B. SEC chromatograms of complexes between zfBG_O and zfBG_O-D1 (A) and zfBG_O and zfBG_O-D2 (B) with TGF-β2DM and TβRII are depicted in blue and red, respectively. Chromatograms of the TGF-β2DM:TβRII binary complex and zfBG_O-D1/zfBG_O-D2 alone are shown in purple and orange curves, respectively. C, D. SPR sensorgrams for binding of zfBG_O-D1 (C) and zfBG_O-D2 (D) to immobilized TGF-β2. Kinetic fit is shown in red over the experimental data shown in black. SPR sensor chip with immobilized TGF-β2 is the same as that used for the experiments shown in Figs 2 and S2. E-F. Methyl region of natural abundance ¹H-¹³C HSQC spectra of BG_O-D1 (E) and BG_O-D2 (F).