Table 3.

SPR binding constants for receptor binding to TGF-β2 or mmTGF-β2-7M

Surface	Analyte	ka1 (M−1 s−1)	kd1 (s−1)	KD1 (μM)	ka2 (M−1 s −1)	kd2 (s−1)	KD2 (μM)	Rmax1 (RU)	Rmax2 (RU)	km (m/s)1
TGF-β2	zfBGO	5.28±0.04 × 105	7.72 ± 0.08 × 10−3	0.015 ± 0.002	n.d.2	n.d.	n.d.	181±9	n.d.
TGF-β2	rBGO	3.74±0.07 × 103	3.45±0.05 × 10−3	0.009 ± 0.002	n.d.	n.d.	n.d.	212±6	n.d.
mmTGF-β2-7M	TβRII	2.03± 0.03 × 106	1.28±0.03 × 10−1	0.063± 0.001	n.d.	n.d.	n.d.	71±5	n.d.
TGF-β2	zfBGO-D1	9.3±0.3 × 103	0.114±0.004	12.3±0.4	n.d.	n.d.	n.d.	20.8±0.4	n.d.	1.2±0.3 × 106
TGF-β2	zfBGO-D2	5.2±0.3× 103	0.0064±0.0017	1.2±0.3	2.8±0.7 × 105	2.7±3.9 × 103	0.0083±0.0100	18±0.3	1.8±0.3

¹K_m, mass transfer rate constant

²n.d., not determined