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. Author manuscript; available in PMC: 2020 Sep 3.
Published in final edited form as: Structure. 2019 Jul 11;27(9):1384–1394.e4. doi: 10.1016/j.str.2019.06.006

Figure 1. BBSome subunits and cryo-EM density map of the BBSome.

Figure 1.

A. Domain organization of the eight BBSome subunits. The 29 domains making up the BBSome subunits are 4 β-propellers (BBS1/2/7/9), one 4-helix bundle inserted into a β-propeller (BBS1), 4 connector helices between the β-propellers and the GAE domains (some of which are predicted to form coiled coils) (BBS1/2/7/9), 4 GAE domains (BBS1/2/7/9), 3 platform domains (BBS2/7/9), 3 hairpins (BBS2/7/9), 3 helical bundles (BBS2/7/9), 3 α-solenoids (BBS4/8TPR1-2/8TPR3-13), 2 PH domains (BBS5), one 3-helix bundle (BBS5), and one helical micropeptide (BBS18). B. Silver-stained 4-12% SDS-PAGE gel of the BBSome purified from retinal extract. C. Density map of the BBSome at 4.9-Å resolution obtained by single-particle cryo-EM (Map 1), showing a prominent helical bundle that is located in between the base and the top hemisphere.