KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| E. coli Rosetta2(DE3)-pLysS | Millipore Sigma | 70956 |
| Biological Samples | ||
| Bovine retina | W.L. Lawson | Custom order |
| Experimental Models | ||
| E. coli Rosetta2(DE3)-pLysS | Millipore Sigma | 70956 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BS3 | Thermo Scientific | A39266 |
| DSSeb | Proteochem | c1136 |
| Sequencing-grade modified trypsin | Thermo Scientific | PI90305 |
| Deposited Data | ||
| Cryo-EM map of intact BBSome | This paper | EMD-7839 |
| Cryo-EM map of BBSome missing BBS5 | This paper | EMD-7841 |
| Cα model of BBSome | This paper | PDBDEV_00000018 |
| Coordinates of the BBS1βprop–ARL6GTP complex | (Mourão et al., 2014) | PDB: 4V0M |
| Recombinant DNA | ||
| pGEX6P-ARL6ΔN16[Q73L] | (Jin et al., 2010) | N/A |
| Software and Algorithms | ||
| UCSFImage4 | (Li et al., 2015) | https://cryoem.ucsf.edu/software/UCSFImage.html |
| MotionCor2 | (Zheng et al., 2017) | https://msg.ucsf.edu/software |
| CTFFIND4 | (Rohou and Grigorieff, 2015) | http://grigoriefflab.janelia.org/ctffind4 |
| Relion 1.3 | (Scheres, 2015) | https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| SPARX | (Hohn et al., 2007) | http://sparx-em.org/sparxwiki/SparxWiki |
| UCSF Chimera | (Pettersen et al., 2004) | https://www.cgl.ucsf.edu/chimera/ |
| genesilico | (Kurowski and Bujnicki, 2003) | https://www.genesilico.pl/meta2/ |
| pLink | (Fan et al., 2015; Yang et al., 2012) | http://pfind.ict.ac.cn/software/pLink/index.html |
| hhpred | (Söding, 2005) | https://toolkit.tuebingen.mpg.de/#/hhpred |
| RosettaCM | (Song et al., 2013) | https://www.rosettacommons.org/software |
| Rosetta ab initio | (Bradley et al., 2005) | https://www.rosettacommons.org/software |
| RosettaES | (Frenz et al., 2017) | https://www.rosettacommons.org/software |
| Other | ||
| Quantifoil R1.2/1.3 holey carbon film on copper 200 mesh grids | Electron Microscopy Sciences | Cat #Q250CR-06 |