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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Dev Biol. 2019 Jun 23;454(2):156–169. doi: 10.1016/j.ydbio.2019.06.012

Fig. 3: Optical projection tomography (OPT) and Tree Surveyor analysis of E12.5 Adamts18−/− lungs illustrate less epithelium and branch formation compared to stage-matched Adamts18+/− and wildtype lungs.

Fig. 3:

A-C: Whole-mount immunostaining of stage-matched Adamts18 E12.5 lungs with cytokeratin were scanned with OPT (n = 5; somite range = 49–51). D-F: Three-dimensional skeletal and segmentation models of lungs from A-C generated in Tree Surveyor. G-I: Dendrograms displaying the branching pattern for the lungs from A-C. Each lobe is color-coded: cranial = green, medial = pink, caudal = yellow, accessory = purple, left = blue. J-L: Dendrograms of each lung’s accessory lobe. M: Tree Surveyor global analysis quantification. Error bars are SD and significance was determined by a two-tailed t-test. ** = p ≤ 0.01, *** = p ≤ 0.001. Line = 200μm.