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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Dev Biol. 2019 Jun 23;454(2):156–169. doi: 10.1016/j.ydbio.2019.06.012

Fig. 6: Adamts18−/− kidneys develop double ureters with higher nephron counts.

Fig. 6:

A-H: Whole-mount immunostaining of embryonic kidneys with epithelial marker cytokeratin (white) at E10.5, E11.5, E12.5, and E15.5 illustrating Adamts18−/− kidneys with double ureters are larger than wildtype kidneys. C and D are additionally stained with ureteric bud tip marker Ret (red), demonstrating that all outgrowths from the nephric duct express the tip marker. I, J: Sections of adult kidneys (6 week) stained with the glomerular marker peanut agglutinin (PNA). I: Embryonic Adamts18−/− embryos (E11.5-E15.5) have at least one kidney with a double ureter at a 38% frequency. J: Adults with double ureters have significantly more nephrons based on PNA staining. Dots represent nephron counts per kidney and the black bar is the average. Error bars are SD and significance was determined by a two-tailed t-test. * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001. Line in A-D = 100μm; line in E-H = 200μm, line in I, J = 1000μm.