KEY RESOURCES TABLE.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Ckytokeratin (pan) | Sigma | C2562 |
| Six2 | Proteintech | 11562–1-AP |
| Vimentin | Abcam | ab92547 |
| Ret | Cell Signaling Technology | 3223 |
| Peanut agglutinin | Sigma | L7381 |
| MUC5AC | Novus Biological | NBP2–15196 |
| Prosurfactant Protein C | Millipore | AB3786 |
| Acetyl-alpha tubulin | Millipore | MABT868 |
| Sox9 | Abcam | ab185230 |
| Sox2 | Abcam | ab97959 |
| E-cadherin | BD Biosciences | 610181 |
| Caspase 3 | Novus Biologicals | NBP2–15196 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Critical Commercial Assays | ||
| RNAscope 2.5 Duplex Detection Kit | Advanced Cell Diagnostics | 322430 |
| RNAscope Multiplex Fluorescent v2 Kit | Advanced Cell Diagnostics | 323110 |
| Click-iT EdU Alexa Fluor 647 imaging kit | Thermo Fisher | C10340 |
| RNeasy Micro Kit | Qiagen | 74004 |
| SuperScript IV VILO kit | Thermo Fisher | 1176050 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Mouse: Swiss Webster | Charles River | 024 |
| Mouse: Adamts18−/− | Knock-Out Mouse Project | VG 12442 |
| Mouse: Adamts16−/− | This paper | N/A |
| Oligonucleotides | ||
| Adamts18 in situ probe primers: Forward: acccttgtcctgagaatagcttg Reverse: TAATACGACTCACTATAGGGgaggaattgacttgggtgtgttg |
This paper | N/A |
| Adamts16 in situ probe primers: Forward: caagtggcagtttggagagaaag Reverse: TAATACGACTCACTATAGGGtagtactggttggtggaatggtg |
This paper | N/A |
| Muc5ac qPCR primers: Forward: CAGGACTCTCTGAAATCGTACCA Reverse: AAGGCTCGTACCACAGGGA |
This paper | N/A |
| Sftpc qPCR primers: Forward: ATGGACATGAGTAGCAAAGAGGT Reverse: CACGATGAGAAGGCGTTTGAG |
This paper | N/A |
| Tuba4a qPCR primers: Forward: GAGATCCGAAATGGCCCATAC Reverse: GTGGAATACTAGGAAGCCCTGA |
This paper | N/A |
| Adamts18 (Exon 3–4) qPCR primers: Forward: CCGCAGTGTTTGTATCAGGG Reverse: TACAGGACGTGAGGATGGTG |
This paper | N/A |
| Adamts18 (Exon 10–11) qPCR primers: Forward: GGACAGATTTATGATGCCGACA Reverse: GGTGGCACCAGAGTGACTT |
This paper | N/A |
| Adamts16 (Exon 1–2) qPCR primers: Forward: CTGAGTCCTGTGAGCCCTC Reverse: CACCGGTTTCCAGCCAGC |
This paper | N/A |
| Adamts16 (Exon 17–18) qPCR primers: Forward: ccaaggtcaggggctaagaa Reverse: tcccgcctttgagttcatct |
This paper | N/A |
| Gapdh qPCR primers: Forward: AGGTCGGTGTGAACGGATTTG Reverse: TGTAGACCATGTAGTTGAGGTCA |
This paper | N/A |
| Recombinant DNA | ||
| Software and Algorithms | ||
| Tree Surveyor | Short et al., 2013 | N/A |
| NRecon | Bruker microCT | www.bruker.com |
| Jstree | Heng Li | http://lh3lh3.users.sourceforge.net/jstree.shtml |
| Other | ||
| Adamts18 RNAscope probe | Advanced Cell Diagnostics | 452251 |
| Spry2 RNAscope probe | Advanced Cell Diagnostics | 425061 |
| Shh RNAscope probe | Advanced Cell Diagnostics | 314361-C2 |
| Bmp4 RNAscope probe | Advanced Cell Diagnostics | 401301-C2 |
| Fgf10 RNAscope probe | Advanced Cell Diagnostics | 446371 |