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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Mol Cancer Res. 2019 Jun 19;17(9):1945–1957. doi: 10.1158/1541-7786.MCR-19-0077

Figure 5. KRAS knockdown inhibits EMT and infiltrative behavior of human GA-derived cells.

Figure 5.

(A) Western blot for KRAS and EMT proteins in spheroids of AGS and KATOIII transduced with sh.KRAS or sh.Scr. (B) Immunofluorescence photos of AGS and KATOIII spheroids for DAPI (blue), E-cadherin (green), and Snail (red) following transduction with sh.KRAS or sh.Scr. Graphs show relative expression of CD44 and Snail. Scale bar, 50 μm. (C-D) Migration and invasion assays (C) and soft agar assay (D) for spheroids of AGS and KATOIII transduced with sh.KRAS or sh.Scr. (E) Tumor and H&E pictures of flank xenografts from AGS cells stably transduced with sh.KRAS or sh.Scr, Photos of immunofluorescence staining with infiltrating cells for DAPI (blue) and Snail (green). Dashed line indicates tumor border. Graph showing number of Snail(+) infiltrating cells. (F) Number of Snail(+) cells infiltrating beyond tumor border. Bars represent standard deviation. *p<0.05 compared to control.