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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Cancer Res. 2019 Jul 15;79(17):4426–4438. doi: 10.1158/0008-5472.CAN-19-0237

Figure 4. APE1 silencing or APE1 redox-specific inhibition decreases MMP-14 protein levels.

Figure 4.

Representative immunofluorescent images of MMP-14 (green) and APE1 (red) in FLO-1 or CPB cells; nuclei were stained with DAPI (blue). A, APE1 stable knockdown cells (shAPE1), and their control cells (shCtrl). B, Cells were treated with 40μM E3330 or vehicle control (Ctrl) for 24 h before staining. C, 3D organotypic culture of CPB cells with stable knockdown (shAPE1) or control (shCtrl). Hematoxylin and eosin (H&E) staining of sequential cut of the same blocks used for immunofluorescence staining. The quantification of MMP-14 intensity was expressed as the mean ± SEM of 3 independent fields. *, p < 0.05.