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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Cancer Res. 2019 Jul 15;79(17):4426–4438. doi: 10.1158/0008-5472.CAN-19-0237

Figure 6. APE1 is required for the rapid MMP-14 endocytic recycling in EAC cells.

Figure 6.

A & B, Endocytosis assays of HA-tagged MMP-14 in FLO-1 cells. Cells were transfected with HA-tagged MMP-14. The cell lysates were collected for biotin pulldown at the indicated time points of endocytosis (37°C incubation). C & D, Recycling assays of HA-tag labelled MMP-14 in FLO-1 cells. Recycling assays were performed after 10 min endocytosis (37°C incubation). The cell lysates were collected for biotin pulldown at indicated time courses of recycling (2nd 37°C incubation). APE1-knockdown cells (shAPE1) and control cells (shCtrl) of FLO-1 were used (A, C). FLO-1 cells were pretreated with 40μM E3330 for 24 h before experiments (B, D). Relative endocytic MMP-14 levels were measured by the ratio of endocytic MMP-14/total MMP-14. All quantification analyses were based on independent triplicate experiments. *, p < 0.05; **, p < 0.01.