Figure 4.

Regulation of translation–initiation complex by metformin, and rescue from metformin actions by Methyl-pyruvate. A, 7mGTP pull down and eIF4G co-IP (B) were performed to analyze the regulation of translation–initiation complex formation by mTOR inhibition treated by metformin with indicated doses for 24 hours. B, Methyl-pyruvate (3 mM) was added to the cell culture medium, and rescued the mTOR inhibition induced by metformin (3 mM) and rotenone (3 nM) (24 hour treatment). C, Methyl-pyruvate (3 mM) rescued from metformin- and rotenone-induced decrease in colony formation in the control CAL33 cells.