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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Mol Cancer Res. 2019 May 28;17(9):1920–1930. doi: 10.1158/1541-7786.MCR-18-1291

Figure 4: Mutational analysis of SRSF3 RNA cis-element indicates the pentameric ESE is essential for CPEB2 exon 4 inclusion.

Figure 4:

A. Schematic representation of the mutant ESE minigene. Red bar indicates the position of the RNA cis-element is located near the 5’ splice site. Genomic coordinates indicate the first nucleotide base in the RNA cis-element and were extracted from Genome Reference Consortium Human Build 38 (GRCh38.p12) B. RT-PCR analysis of MDA-MB-231 Par cells for the wild type (WT) CPEB2 ESE compared to mutant (Mut) MG-specific CPA/CPB ratio. C. RT-PCR analysis of MDA-MB-231 AnR cells for the WT CPEB2 ESE compared to Mut MG-specific CPA/CPB ratio. Representative images from 3 independent experiments are shown. All quantitation shown as n = 3 ± standard deviation (sd) via densitometry. Statistical significance is reported as p-value from oneway ANOVA pooled t-test of the MG CPA/CPB ratio. (* = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001).