Table 2. BRAF double kinase was not detected in parental cells.

In order to count reads spanning the putative 18–10 splice junction, as well as canonical splice junctions 9–10, 18–18, and 18–19, we used BLAST to determine short sequences at the relevant end of each exon. RNA seq data from cell lines indicated that were mapped to canonical BRAF or to the BRAF kinase duplication junction.

Total reads	9–10	18–10	18–18	18–19
1205LuTR	75	0	1	35
CRT20	76	29	3	26
CRT21	91	25	8	35
Unique reads	9–10	18–10	18–18	18–19
1205LuTR	58	0	1	31
CRT20	61	22	3	21
CRT21	71	20	8	33