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. 2019 Sep 4;9:12780. doi: 10.1038/s41598-019-49146-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HAC1-mRNA splicing is transiently induced upon diauxic shift of yeast cells. (a) After being cultured for the indicated durations in SD medium or stressed by the canonical ER stressor tunicamycin, IRE1+ cells were analyzed by RT-PCR amplification of the HAC1 transcripts (See Fig. S11 for the uncropped gel image). (b) The same experiment as shown in panel A was performed using three independent clones of IRE1+ cells to obtain quantified data. (c) Optical density of the cultures analyzed in panel B. (d) The same experiment as described in panel A was performed using ire1Δ cells (See Fig. S11 for the uncropped gel image). (e,f) IRE1+ cells were cultured in YPD, and analyzed for HAC1-mRNA splicing and culture density.