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. 2019 Sep 4;9:12780. doi: 10.1038/s41598-019-49146-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ire1-dependent enhancement of respiration and mitochondria size after diauxic shift. IRE1+ cells and ire1Δ cells were cultured in SD medium for the indicated durations. (a) Cell lysates (equivalent to 0.22 OD₆₀₀ cells) were analyzed by anti-Por1 Western blotting. Anti-Pgk1 Western blot serves as a loading control. See Fig. S12 for the uncropped blot image. (b,c) Cells producing mitochondria-located GFP were optically sectioned by a confocal microscope and monitored for fluorescing area. Note that size distribution of IRE1+ cells and that of ire1Δ cells are statistically different (p = 3.0 × 10¹³). (d) Oxygen consumption rates were assayed as described in the Materials and Methods section. (e) Novel activation mode and role of Ire1 presented in this study is illustrated.