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. 2019 Sep 4;9:12757. doi: 10.1038/s41598-019-49287-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The specimen was placed in a Petri dish. (b) The specimen washed with a sterile PBS solution to remove residual blood from the tissue surface. (c) The connective tissues were trimmed away. (d) The tissues were cut into blocks approximately 1 cm in diameter. (e) The connective tissues, bile ducts, and blood vessels were trimmed away as much as possible. These tissue blocks were further chopped into small approximately 1-mm pieces. (f) The mixed-enzyme solution and the liver tissue. (g) The Gentle MACSTM tissue processor is running the h_tumor_01.01 program. (h) Tissue gently digested in the C tube. (i) Single cell suspension of liver tissue.