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. 2019 Sep 4;9:12786. doi: 10.1038/s41598-019-49241-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR data of TIME cells. TIME were stimulated with a cytokine mix consisting of IL-1β, TNF-α and IFN-γ (each in a concentration of 5 ng/ml) and/or supplemented with the unsaturated fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6). Quantification of miRNA expression was performed by means of the miRCURY LNA SYBR Green PCR kit and appropriate miRCURY LNA miRNA PCR assay primers on a CFX96 Touch Real Time PCR detection system (N = 4, n = 2). The fluorescence signal of all samples was measured at the end of every PCR cycle as relative fluorescent unit (RFU). miRNA expression is displayed as mean difference to unstimulated control on the basis of raw Ct values. The mean RFU values and standard deviations are plotted as a sigmoidal curve.