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. 2019 Sep 4;9:12786. doi: 10.1038/s41598-019-49241-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ddPCR data of TIME cells. TIME were stimulated with a cytokine mix consisting of IL-1β, TNF-α and IFN-γ (each in a concentration of 5 ng/ml) and/or supplemented with the unsaturated fatty acids docosahexaenoic acid (DHA, C22:6n3) or arachidonic acid (AA, C20:4n6). Absolute quantification of miRNA expression was performed by means of the EvaGreen Supermix and appropriate miRCURY LNA miRNA PCR assay primers on a QX200 ddPCR droplet reader system (N = 4, n = 2). miRNA expression was measured as RNA molecules per µl sample. Results are displayed as fold change to unstimulated control.