Fig. 1.

Molecular phylogenetic tree of Cx26, Cx30 and Cx32 genes. Bayesian Inference on amino acid sequences belonging to the main vertebrate lineages. Numbers close to nodes indicate posterior probability values (>0.95). Amia Calva GEUG01003334.1 (Cx31-like) was used as the outgroup. If the species name is red, the sequence possesses the carbamylation motif. The green box indicates Cx26 with a short C-terminal tail. The Cx27.5 sequence for lamprey is in purple as it has elements of the carbamylation motif but is very different from any other motif (see text for discussion). Two main clades are apparent and labelled A and B