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. 2019 Sep 4;2:331. doi: 10.1038/s42003-019-0576-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Whole-cell patch clamp recordings demonstrate a CO₂-sensitive conductance in HeLa cells expressing Chelonia and Gekko Cx26, but not in untransfected parental HeLa cells or HeLa cells expressing Xenopus Cx26. Red bar indicates application of 55 mmHg hypercapnic saline. Cells clamped at −50 mV, with steps to −40 mV to assess whole-cell conductance. KW, Kruskal–Wallis ANOVA. Box and whisker plots: box, first and third quartiles; horizontal line, median; whiskers, the furthest point that lies no more than 1.5 times the interquartile range from the median. Each point represents an independent patch clamp recording (replicate)