Fig. 7.

Cx32 hemichannels in fish can be opened by high levels of CO₂. a Sequence of the carbamylation motif (pink box) and extended C-terminal tail in Cx32 of human, Danio and Rhincodon. Note the presence of proline residues in the extended C-terminal tails of each species. b Dye loading experiments showing a small amount of dye loading with 55 mmHg CO₂, and substantial loading with 70 mmHg PCO₂ and zero Ca²⁺. c Box and whisker plots: box, first and third quartiles; horizontal line, median; whiskers, the furthest point that lies no more than 1.5 times the interquartile range from the median. All points the median change in pixel intensity from the 35 mmHg control saline. Each point represents result from an independent transfection. Scale bar 20 µm