Table 1.
Primers used in this study
| Construct/mutation | Substrate | Forward primer | Reverse primer |
|---|---|---|---|
| xtCx26Δ | Xenopus pCAG-Xenopus-mCherry | ATTCGGTACCATGGATTGGGGAACG | XTHLrev2, CTCTGGTACCCCTGAATGTTTTTTTGACCTCCTTAGGGAAGC |
| xtCx26ΔPV | Xenopus pCAG-Xenopus-mCherry | ATTCGGTACCATGGATTGGGGAACG | Xen244PVrev, GGTATGGTACCCCaactggCTTTTTTGACCTCCTTAGGGAAGC |
| hCx26 + XenCT | pCAG-humCx26-mCherry | mchmid, AGGACGGCGAGTTCATCTAC | hum-Xen, agaatgcctgagtgaatgtttTTTTGACTTCCCAGAACAATATC |
| insert PCR fragment | pCAG-Xenopus-mCherry | XenTail, tattgttctgggaagtcaaaaAAACATTCACTCAGGCATTCTAACC | mch207, GGTGCTTCACGTAGGCCTTGGAGCCGTACATGAAC |
| P228G/P229G | human Cx32 | ccgagcccagcgccgctccaatGGCGGttcccgcaaggg | cccttgcgggaaccgccattggagcggcgctgggctcgg |
| P242G | human Cx32 | ttcggccaccgcctctcaGGtgaatacaagcag | ctgcttgtattcacctgagaggcggtggccgaa |
| P268G | human Cx32 | catactgcgccgcagcGGtggcaccgggg | ccccggtgccaccgctgcggcgcagtatg |
| G222P | Lepidosiren paradoxa Cx26 | ataaaggcatgtctccgtcatCCTaaacaggaaaagtactcgagc | gctcgagtacttttcctgtttaggatgacggagacatgcctttat |
| G238P | Lepidosiren paradoxa Cx26 | gctcccagtcaagtttaatgtctaaaCCcaaagagcatcagca | tgctgatgctctttgggtttagacattaaacttgactgggagc |
Primer names mentioned in the Methods section are shown in bold. All primers shown in the 5' to 3' direction.