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HPLC condition used in this study.
| Instrument | Condition |
|---|---|
| Reverse phase-column | CAPCELL PAK C18 (4.6 mm × 25 cm, 5 µm) |
| Oven temp. (°C) | 35 |
| Flow rate (ml/min) | 1 |
| Mobile phase (%) | 38% solvent A: H2O + 0.5% formic acid; 62% solvent B: acetonitrile |
| UV Absorbance (nm) | 265 |
| Gradient elution | isocratic elution |
| Run time (min) | 30–40 |
