Figure 3.

Overexpression of KIFC1 promoted the proliferation of BC cells in vitro. A, Expression levels of KIFC1 protein in normal bladder cell SV‐HUC‐1 and seven BC cell lines by western blotting. Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was adopted as the loading control. B, Western blotting revealed that KIFC1 was stably knocked down or overexpressed in corresponding BC cells. C and D, The proliferation ability of stably transfected BC cells were determined by CCK‐8 and colony forming assays. sh, short hairpin RNA; *P < .05; **P < .01