Figure 5.

KIFC1 could induce the EMT course of BC. A, Relative mRNA expressions of epithelial markers (E‐cadherin, α‐catenin and β‐catenin), mesenchymal markers (vimentin, fibronectin and N‐cadherin) and EMT‐related transcription factors (Snail and Slug) were compared by qPCR between KIFC1‐OE, KIFC1‐silenced, and their corresponding control BC cells. B, Western blotting compared KIFC1 ectopic expression BC cells with their respective control cells in relative expression of EMT protein. C, Immunofluorescence staining showed an upregulated expression of epithelial marker E‐cadherin and a downregulated expression of mesenchymal marker vimentin in TCCSUP‐shKIFC1 cell. Opposite results were seen in T24‐KIFC1‐OE cells. Nuclei were counterstained with DAPI. (magnification: ×600)