Figure 6.

KIFC1 activated Akt/GSK3β signaling to induce proliferation and EMT of BC. A, The expression of total Akt, pAkt, total GSK3β and pGSK3β were detected by western blotting. GAPDH was used as the loading control. B, Western blotting analysis indicated that PI3K inhibitor LY294002 (LY) could effectively decrease expression levels of p‐AKT, p‐GSK3β and Snail induced by KIFC1. C‐F, Wound‐healing and transwell migration assay showed that LY could inhibit the migration ability of KIFC1‐OE cells, and CCK‐8 and colony formation assays revealed the suppressive effect in proliferation of BC cells of LY. *P < .05