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. 2019 Aug 14;110(9):2960–2972. doi: 10.1111/cas.14131

Figure 4.

Figure 4

Circular RNA circCRIM1 suppresses cell metastasis through the circCRIM1‐microRNA (miR)‐182/93‐leukemia inhibitory factor receptor (LIFR) axis. A,B, FISH (A) and separation of cytoplasm and nucleus fraction RNA analysis (B) show that circCRIM1 predominantly resides in the cytoplasm of lung adenocarcinoma cells. C, Pull‐down assay using biotin‐coupled miR‐182, miR‐93, miR‐1205, and miR‐557. D, Through bioinformatics analysis, hsa‐miR‐93 and hsa‐miR‐182 were predicted to be the targets of circCRIM1. E,F, RIPA using an Ab against Ago2. Input was used as positive control. G,H, Luciferase activities of WT LIFR 3′‐UTR and mutant LIFR 3′‐UTR after transfection with miR‐182‐5p mimic and miR‐93‐5p mimic. I, Quantitative real‐time PCR analysis of the expression of LIFR in A549 lysates after biotin‐coupled miR‐182 and miR‐93 pull‐down assays. J, circCRIM1 is positively correlated with LIFR in the expression analysis of 40 lung adenocarcinoma patients. K, Quantitative real‐time PCR of the expression levels of LIFR. L, Western blot analysis of the expression levels of LIFR after cotransfection with si‐circCRIM1, circCRIM1 overexpression plasmid, and miR‐182 and miR‐93 mimics. NC, negative control